Table 1.

Protein expression conditions

Enzyme	Vector	Strain	IPTG (mM)	Temperature (°C)	Time (hr)
rDl3βHSD	pQE30	M15[pREP4]	0.1	4	96
rCt3KSI	pDEST17	BL21 (DE3)	0.4	30	16
rAtSt5βR	pQE30	M15[pREP4]	1.0	37	4
rAtSt5βR_DMa	pQE30	M15[pREP4]	1.0	37	4
rAtSt5βR_DMCb	pQE30	M15[pREP4]	1.0	37	4
rAtSt5βR_R64D	pQE30	M15[pREP4]	1.0	37	4

^a AtSt5βR_DM: AtSt5βR_F342A_F153A.

^b AtSt5βR_DMC:AtSt5βR_R64D_F342A_F153A.