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Workflow of American Gut Project data processing
| Steps | Contents |
|---|---|
| Step 1 | Downloaded 19,327 samples (25 Jan. 2018) |
| Step 2 | Excluded non‐fecal samples: 15,259 fecal samples left |
| Step 3 | 12,127 fecal samples with over 10,000 reads |
| Step 4 | 11,744 samples passed the quality control of DADA2 |
| Step 5 | Delete the ASV with a distribution of less 1% and not belong to bacteria |
| Step 6 | Delete blooming bacteria |
| Step 7 | Excluded samples with diseases |
