Figure 6.

Spa33^C interact with MxiC and MxiI and forms a complex in vitro. (a) Analysis of the clarified lysates (LY) of His‐Spa33‐His or Spa33^C‐His and His‐MxiC by immuno‐detection using anti‐his antibodies. Analysis of the eluted fractions (EF) by Coomasie blue staining (upper panels) and immuno‐detection using anti‐his antibodies (lower panels) of (b) GST‐MxiC or GST alone against His‐Spa33‐His or Spa33^C‐His. (c) GST‐MxiI or GST alone against His‐Spa33‐His or Spa33^C‐His and (d) GST‐MxiI or GST alone against both His‐Spa33‐His and His‐MxiC. Mw: molecular weight in kDa