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Purification of Spa33‐His and Spa33C‐His in E. coli. Spa33‐His and Spa33C‐His were over expressed in E. coli (Top10) and purified using His Cobalt Resin. Eluted fractions (EF) were separated on 15% SDS‐PAGE and visualized by Coomassie blue staining. Spa33CC was excised from the gel and analyzed by MS. Mw: molecular weight in kDa
