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Whole cell extracts (WCE) of the wild‐type strain (WT), the spa33 mutant (∆spa33), the spa33 mutant complemented by pSU18‐Spa33 (pSpa33) or its mutated derivatives, with or without Spa33C (pSpa33C), analyzed by Western blot using anti‐IpaC, IpaB IpaD, MxiC, IpaA, IscB, OspD1, IpaH, and anti‐His antibodies. *Nonspecific band
