Table A1.
List of plasmids and strains used in this study
| Description | Source | |
|---|---|---|
| Strains | ||
| S. flexneri M90T | S. flexneri strain is a derivative of the wild‐type strain M90T (serotype 5) which is resistant to streptomycin | Allaoui, Sansonetti, and Parsot (1993) |
| MxiC‐ | mxiC deficient mutant derived from S. flexneri M90T (serotype 5) | Botteaux et al. (2009) |
| MKA1 | spa33 mutant derived from S. flexneri M90T(serotype 5) | This study |
| MKA2 | mxiCspa33 double mutant derived from S. flexneri M90T (serotype 5) | This study |
| Plasmids | ||
| pKD46 | Plasmid used for expression of the λ Red system | Datsenko and Wanner (2000) |
| pMK1 | pSU18Spa33 (His‐tag fused at COOH‐termini) | This study |
| pMK2 | pSU18MxiCSpa33 (His‐tag fused at COOH‐termini) | This study |
| pMK3 | pSU18Spa33GTC (His‐tag fused at COOH‐termini) | This study |
| pMK4 | pSU18Spa33V192A (His‐tag fused at COOH‐termini) | This study |
| pMK5 | pSU18Spa33V192D (His‐tag fused at COOH‐termini) | This study |
| pMK6 | pSU18MxiCSpa33GTC (His‐tag fused at COOH‐termini) | This study |
| pMK7 | pSU18MxiCSpa33V192A (His‐tag fused at COOH‐termini) | This study |
| pMK8 | pSU18MxiCSpa33V192D (His‐tag fused at COOH‐termini) | This study |
| pMK9 | pSU18MxiCF206SSpa33 (His‐tag fused at COOH‐termini) | This study |
| pMK10 | pSU18MxiCF206SSpa33V192D (His‐tag fused at COOH‐termini) | This study |
| pYC1 | pSU18MxiCMxiH | Cherradi et al. (2013) |
| pAB30 | pGEX4t1MxiC (GST‐tag fused at NH2‐termini) | Botteaux et al. (2009) |
| pAB117 | pGEX4t1MxiCF206S (GST‐tag fused at NH2‐termini) | Cherradi et al. (2013) |
| pYC178 | pET30aMxiC (His‐tag fused at NH2‐termini) | Cherradi et al. (2013) |
| pSL24 | pGEX4t1MxiI (GST‐tag fused at NH2‐termini) | Cherradi et al. (2013) |
| pMK11 | pBADSpa33C (His‐tag fused at COOH‐termini) | This study |
| pMK15 | pGEX4T1Spa33 (GST‐tag fused at NH2‐termini) | This study |
| pMK16 | pGEX4T1Spa33V192D (GST‐tag fused at NH2‐termini) | This study |
| pMK17 | pGEX4T1Spa33C (GST‐tag fused at NH2‐termini) | This study |
| pMK18 | pGEX4T1Spa33CHis (GST‐tag fused at NH2‐termini and His‐tag fused at COOH‐termini) | |
| pMK19 | pET30aSpa33his (His‐tag fused at both NH2‐ and COOH‐termini's) | This study |
| pMK20 | pET30aSpa33 (His‐tag fused at NH2‐termini) | This study |
| pMK21 | pET30aSpa33GTChis (His‐tag fused both at NH2‐ and COOH‐termini's) | This study |
| pMK22 | pET30aSpa33V192Dhis (His‐tag fused both at NH2‐ and COOH‐termini's) | This study |
| pMK23 | pET30aSpa33V192Ahis (His‐tag fused both at NH2‐ and COOH‐termini's) | This study |
| pMK24 | pET30aSpa33Slippage (mutated slippage site with silent mutation | This study |
| pMK25 | pSU18MxiCSpa33Slippage (mutated slippage site with silent mutation) | This study |
| pMK26 | pBADSpa33 (His‐tag fused at COOH‐termini) | This study |
| pMK31 | pBADSpa33STOP (His‐tag fused at COOH‐termini) | This study |
| pMK32 | pBADHisSpa33 (His‐tag fused at NH2‐termini) | This study |