Figure 5.

HRM assay 1 (Landolt et al., 2019) allows the distinction between M. tuberculosis/M. canettii/M. africanum/M. orygis/M. pinnipedii/Clade A1, M. microti/M. canettii (rare subtype), and M. bovis/M. bovis BCG/M. caprae/rare M. caprae/M. bovis ecotypes. A combination of HRM assays 1 and 2 is leading to six groups (M. tuberculosis/M. canettii, M. africanum/M. orygis/M. pinnipedii/Clade A1, M. microti, M. canettii (rare subtype), M. caprae/rare M. caprae/M. bovis ecotypes I and II, and M. bovis/M. bovis BCG). By performing HRM assay 3, M. bovis, M. bovis BCG, and rare M. caprae/M. bovis ecotype I can further be distinguished. 1. M. africanum not distinguishable in gyrA and gyrB from M. orygis, M. pinnipedii, Clade A1 (Dassie bacillus, M. mungi, Chimpanzee bacillus, M. suricattae) (Brites et al., 2018). 2. Frequent subtype, intrinsic pyrazinamide (PZA) resistance. 3. Rare ecotypes, no intrinsic PZA resistance. 4. Rare subtype, highly recombinogenic