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Primers used for the three‐reaction HRM paradigm
| Assay | Primer | Target | Amplicon | Primer sequence (5′ → 3′) | Reference |
|---|---|---|---|---|---|
| HRM assay 1 |
HRM_gyrB_for HRM_gyrB_rev |
gyrB | 144 bp |
CGGCTCGAAGTCGAGATCAAG TTCGAAAACAGCGGGGTCG |
Landolt et al. (2019) |
| HRM assay 2 |
HRM_gyrB2_for HRM_gyrB2_rev |
gyrB | 107 bp |
CAAATCGTTTGTGCAGAAGGTCTG CTTGCGCCGAGGACACAG |
This study |
| HRM assay 3 |
HRM_gyrA_for HRM_gyrA_rev |
gyrA | 107 bp |
AGGCAATCCTGGACATGCAG GATGTCTTCCAGATCGGCGATC |
This study |
