Table 3.

Intra‐ and interassay variability of HRM assay 2 of cultured samples

	Run 1		Run 2		Run 3		Interassay
	Tm	CV%	Tm	CV%	Tm	CV%	Tm	CV%
M. tuberculosis H37Rv	83.55		83.75		83.6		83.63	0.12
M. bovis BCG Pasteur ATCC 35734	82.62		82.77		82.70		82.7	0.09
M. microti ATCC 19422	83.13		83.25		83.28		83.22	0.10
M. tuberculosis (n = 3)	83.56 ± 0.04	0.01	83.84 ± 0.04	0.02	83.66 ± 0.06	0.04	83.70 ± 0.18	0.17
M. bovis (n = 6)	82.70 ± 0.10	0.04	82.93 ± 0.08	0.03	82.74 ± 0.06	0.02	82.80 ± 0.20	0.14
M. caprae (n = 6)	83.12 ± 0.09	0.03	83.27 ± 0.09	0.02	83.23 ± 0.06	0.02	83.19 ± 0.16	0.11
M. microti (n = 7)	83.10 ± 0.08	0.02	83.34 ± 0.09	0.04	83.29 ± 0.04	0.01	83.23 ± 0.21	0.14

Mean values and standard deviation of melting temperatures (T_m) of a randomly chosen subset of cultured samples are listed. Corresponding coefficients of variation (CV) in % are indicated for each MTBC species tested.