Table A3.

Raw data set and statistical parameters generated from the intra‐ and interassay variability of high‐resolution melting (HRM) assay 2 using a randomly chosen subset of 22 cultured samples

Isolate			Run 1						Run 2						Run 3						Inter‐Assay
MTBC member	Sample no	Origin	Ct	Tm	Mean Tm	SD	CV%	Intra‐assay CV%	Ct	Tm	Mean Tm	SD	CV%	Intra‐assay CV%	Ct	Tm	Mean Tm	SD	CV%	Intra‐assay CV%	Mean Tm	SD	CV%	Inter‐assay CV%
M. microti	ATCC 19422		20.65	83.13					20.87	83.25					19.74	83.28					83.22	0.08	0.10
M. bovis BCG Pasteur	ATCC 35734		24.67	82.62					24.82	82.77					23.57	82.70					82.70	0.08	0.09
M. tuberculosis	H37RV		22.41	83.55					22.44	83.75					20.97	83.60					83.63	0.10	0.12
M. tuberculosis	15‐961 2B	Elephant	14.87	83.60	83.58	0.02	0.02		15.11	83.80	83.80	0.00	0.00		14.01	83.70	83.69	0.02	0.02		83.69	0.11	0.13
			14.95	83.58					15.13	83.80					14.05	83.70
			14.96	83.57					15.14	83.80					13.94	83.67
M. tuberculosis	15‐1115‐2	Elephant	16.34	83.55	83.54	0.01	0.01		16.69	83.87	83.86	0.03	0.03		15.32	83.63	83.65	0.03	0.03		83.69	0.16	0.19
			16.71	83.55					16.65	83.83					15.47	83.65
			16.73	83.53					16.75	83.88					15.46	83.68
M. tuberculosis	15‐1221‐1	Elephant	19.59	83.53	83.53	0.01	0.01	0.01	19.98	83.87	83.83	0.04	0.04	0.02	18.45	83.60	83.67	0.06	0.07	0.04	83.68	0.15	0.18	0.17
			19.80	83.52					20.02	83.83					18.45	83.68
			19.77	83.53					19.75	83.80					18.47	83.72
M. caprae	229171	Cow	20.74	83.05	83.05	0.00	0.00		21.20	83.23	83.24	0.01	0.01		19.79	83.23	83.23	0.02	0.02		83.17	0.11	0.13
			37.30						21.09	83.25					19.76	83.22
			21.07	83.05					21.28	83.23					19.55	83.25
M. caprae	22914	Cow	20.60	83.10	83.12	0.03	0.03		20.97	83.30	83.26	0.04	0.04		19.52	83.27	83.25	0.02	0.02		83.21	0.08	0.10
			20.68	83.10					21.02	83.25					19.59	83.25
			20.71	83.15					20.79	83.23					19.48	83.23
M. caprae	22848	Cow	22.93	83.07	83.04	0.02	0.03		23.24	83.20	83.19	0.01	0.01		21.68	83.23	83.20	0.03	0.04		83.14	0.09	0.10
			22.94	83.03					23.20	83.18					21.77	83.20
			22.90	83.03					23.34	83.18					21.53	83.17
M. caprae	22966	Cow	19.89	83.13	83.15	0.02	0.02		20.04	83.35	83.33	0.04	0.05		18.60	83.25	83.26	0.02	0.02		83.25	0.09	0.11
			19.84	83.15					19.97	83.35					18.46	83.25
			19.81	83.17					20.04	83.28					18.84	83.28
M. caprae	13‐450	Cow	18.04	83.20	83.16	0.04	0.04		18.23	83.30	83.31	0.02	0.02		17.00	83.28	83.28	0.01	0.01		83.25	0.08	0.09
			18.04	83.13					18.10	83.33					16.99	83.28
			18.13	83.15					18.21	83.30					16.93	83.27
M. caprae	13‐162	Cow	22.08	83.13	83.10	0.03	0.03	0.03	22.43	83.23	83.24	0.01	0.01	0.03	20.91	83.25	83.26	0.01	0.01	0.02	83.20	0.08	0.10	0.11
			21.93	83.10					22.23	83.25					20.75	83.25
			22.01	83.08					22.33	83.23					20.87	83.27
M. bovis	20593	Cow	26.50	82.65	82.64	0.01	0.01		27.13	82.88	82.93	0.06	0.08		25.69	82.68	82.69	0.01	0.01		82.75	0.15	0.18
			24.89	82.65					23.85	83.00					24.93	82.70
			25.63	82.63					24.38	82.90					25.46	82.70
M. bovis	20594	Cow	25.56	82.60	82.61	0.02	0.02		26.03	82.88	82.88	0.03	0.03		24.80	82.72	82.71	0.02	0.03		82.73	0.14	0.16
			24.81	82.60					25.75	82.85					24.77	82.72
			25.43	82.63					25.79	82.90					23.95	82.68
M. bovis	20175	Cow	18.77	82.80	82.74	0.05	0.06		18.90	82.98	82.93	0.07	0.09		17.68	82.80	82.78	0.03	0.03		82.82	0.10	0.12
			18.80	82.70					18.92	82.97					17.53	82.80
			18.81	82.73					18.94	82.85					17.66	82.75
M. bovis	20596	Cow	21.91	82.67	82.67	0.08	0.09		22.37	82.95	82.94	0.01	0.01		21.14	82.80	82.76	0.04	0.04		82.79	0.14	0.17
			22.42	82.75					22.47	82.93					20.81	82.75
			22.56	82.60					22.43	82.95					21.01	82.73
M. bovis	22667	Cow	19.91	82.80	82.80	0.00	0.00		20.04	82.93	82.92	0.01	0.01		18.95	82.78	82.78	0.01	0.01		82.83	0.08	0.10
			19.84	82.80					20.05	82.92					18.79	82.77
			19.81	82.80					20.05	82.92					19.09	82.78
M. bovis	22539	Cow	21.42	82.73	82.76	0.04	0.04	0.04	21.80	82.92	82.92	0.00	0.00	0.04	20.49	82.77	82.76	0.01	0.01	0.02	82.81	0.09	0.11	0.14
			21.55	82.75					21.44	82.92					20.44	82.75
			21.38	82.80					21.60	82.92					20.52	82.75
M. microti	22928	Cat	16.90	83.17	83.17	0.02	0.02		17.13	83.40	83.42	0.02	0.02		16.01	83.30	83.31	0.01	0.01		83.30	0.13	0.15
			16.93	83.18					17.13	83.43					15.89	83.30
			17.01	83.15					17.08	83.43					16.13	83.32
M. microti	15‐1765	Cat	21.77	83.18	83.16	0.03	0.03		22.21	83.32	83.30	0.02	0.02		20.79	83.28	83.28	0.02	0.02		83.25	0.08	0.09
			21.44	83.17					21.93	83.30					19.99	83.27
			21.78	83.13					22.05	83.28					20.59	83.30
M. microti	14‐58	Cat	24.40	83.02	83.02	0.01	0.01		24.39	83.28	83.27	0.02	0.02		23.44	83.28	83.28	0.02	0.02		83.19	0.15	0.18
			24.39	83.02					24.64	83.28					23.18	83.30
			24.31	83.03					24.43	83.25					23.45	83.27
M. microti	14‐690	Alpaca	15.10	83.05	83.05	0.03	0.03		15.33	83.30	83.29	0.01	0.01		14.28	83.30	83.29	0.01	0.01		83.21	0.14	0.17
			15.13	83.07					15.27	83.28					14.20	83.30
			15.21	83.02					15.29	83.30					14.24	83.28
M. microti	16‐2156	Cat	23.07	83.07	83.08	0.02	0.02		23.48	83.30	83.31	0.01	0.01		22.32	83.27	83.27	0.00	0.00		83.22	0.12	0.14
			23.49	83.08					23.60	83.30					22.17	83.27
			23.49	83.10					23.57	83.32					22.01	83.27
M. microti	1522744	Cat	22.79	83.12	83.09	0.03	0.03		22.89	83.33	83.29	0.03	0.04		21.82	83.27	83.26	0.01	0.01		83.21	0.11	0.13
			22.67	83.07					22.97	83.27					21.74	83.27
			22.68	83.08					22.94	83.27					21.49	83.25
M. microti	16‐1347	Cat	18.92	83.13	83.15	0.03	0.03	0.02	19.20	83.30	83.27	0.03	0.03	0.02	17.52	83.30	83.30	0.00	0.00	0.01	83.24	0.08	0.10	0.14
			18.94	83.13					18.99	83.25					17.21	83.30
			18.87	83.18					18.86	83.25					17.32	83.30