Table 1.
Primer sets designed for C. pecorum LAMP and qPCR
| Purpose | Primer name | Nucleotide sequence (5′–3′) | Source | GenBank accessiona |
|---|---|---|---|---|
| LAMP assay | F3 | TATCGTGATCCTGCACATTG | This study | CP004035 |
| B3 | CGCATTGCAATTACAGAAGG | This study | (6,925–7,112) | |
| FIP | TCCCGAAAGCACAGGAGAATTCGCTCGTGTTGGGTAGATG | This study | ||
| BIP | AAGGTGCTAGTTGGTCTTGTGGCTTCATGCCTTCATCCGTAA | This study | ||
| LoopF | TCTTTACTCCTTTGTCCTTCCC | This study | ||
| LoopB | AAGCAATCTCGTGTACGGTT | This study | ||
| qPCR | OmpB‐F | CCAAGCATAATCGTAACAA | Hulse et al., 2018 | U56924 (125–265) |
| OmpB‐R | CGAAGCAAGATTCTTGTC | Hulse et al., 2018 | ||
| OmpB‐Cy5 | Cy5‐ACTTGTTGGCAATTCTTCTCTTCACA | Hulse et al., 2018 | ||
| OmpA‐F | ATGAAAAAACTCTTAAAATCGG | Kollipara et al., 2013 | NC_015408 | |
| OmpA‐R | TTAGAATCTGCATTGAGCAG | Kollipara et al., 2013 | (56741–57916) |
a
Numbers in parentheses indicate nucleotide position.