Table A2.

Specificity of C. pecorum LAMP assay against real‐time PCR C. pecorum OmpA and OmpB target genes

Sample number	Koala swab sample	C. pecorum LAMP		C. pecorum OmpA (real‐time PCR)	C. pecorum OmpB (real‐time PCR)
		Time to amplification	Melt temperature (°C)	Cycling threshold	Cycling threshold
78,729	Left conjunctival	0:00	0.00	0	0
	Right conjunctival	10:00	84.97	23.22	29.06
	Urogenital	10:30	85.40	22.06	23.98
78,732	Left conjunctival	0:00	0.00	0	0
	Right conjunctival	0:00	0.00	0	0
	Urogenital	15:00	85.15	26.01	32.71
78,661	Urogenital	11:00	85.15	35.04	34.51
	Rectal	13:45	84.96	28.46	33.78
71,942	Left conjunctival	0:00	0.00	0	0
	Right conjunctival	0:00	0.00	0	0
	Urogenital	0:00	0.00	0	0
72,035	Urogenital	17:08	84.56	18.16	22.41
72,097	Left conjunctival	0:00	0.00	0	0
78,861	Pooled conjunctival	0:00	0.00	0	0
	Urogenital	10:45	85.07	25.23	33.09
78,822	Pooled conjunctival	13:45	84.76	24.31	25.51
	Urogenital	10:15	84.96	25.59	29.45
79,193	Pooled conjunctival	0:00	0.00	0	0
	Urogenital	0:00	0.00	0	0
	Rectal	0:00	0.00	0	0
71,499	Left conjunctival	9:30	84.51	24.36	28.57
	Right conjunctival	12:00	84.32	23.84	31.21
	Urogenital	16:30	84.61	31.43	30.31
71,494	Left conjunctival	0:00	0.00	0	0
	Urogenital	7:30	84.42	22.65	27.98
71,586	Left conjunctival	9:45	84.66	20.61	27.95
	Right conjunctival	14:00	84.77	22.59	27.31
	Urogenital	15:30	85.00	20.76	29.44
71,257	Left conjunctival	14:15	84.86	18.79	22.40
	Right conjunctival	7:15	84.97	17.98	20.66
	Urogenital	6:15	84.87	15.47	19.10
71,472	Left conjunctival	0:00	0.00	0	0
	Right conjunctival	0:00	0.00	0	0
	Urogenital	18:45	85.26	31.25	25.69
71,719	Left conjunctival	16:30	84.36	27.95	23.82
71,720	Left conjunctival	0:00	0.00	0	0
	Right conjunctival	17:03	84.70	30.55	32.40
	Urogenital	16:45	84.56	20.85	27.83
78,931	Left conjunctival	17:30	84.56	26.05	27.99
	Right conjunctival	12:45	84.17	24.29	27.00
	Urogenital	18:15	84.27	30.83	28.00
	Rectal	0:00	0.00	0	0
78,858	Urogenital	0:00	0.00	0	0