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. 2019 Dec 20;9:19540. doi: 10.1038/s41598-019-55995-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CAP2/Tmprss4 mRNA expression is upregulated by low dietary K⁺ in distal tubules, and also localizes to the papillary transitional epithelium. Relative mRNA transcript expression levels of CAP2/Tmprss4 in (A) kidney, and (B) colon from wildtype mice under regular K⁺ diet (n = 4, triangles) and low K⁺ diet (n = 4, diamonds). (C) Detection of wildtype CAP2/Tmprss4 mRNA transcript expression in microdissected nephron segments (n = 4–6/segment) on regular (RK) and low (LK) potassium diet. PCT: proximal convoluted tubule, PST S3: proximal straight tubule segment 3, mTAL: medullary thick ascending limb of Henle’s loop, cTAL: cortical thick ascending limb of Henle’s loop, DCT: distal convoluted tubule, CNT: connecting tubule, CCD: cortical collecting duct, OMCD: outer medullary collecting duct. (D) RNAscope detection of CAP2/Tmprss4 in renal cortex, medulla and papilla of wildtype mice following LK diet. (E) Negative control for CAP2/Tmprss4 RNAscope detection in knockout (∆/∆) kidney under low K⁺ diet, and negative control for RNAscope fluorescent channels (including channels shown in Fig. 3E,F) in kidney sections from wildtype mice under low K⁺ diet. Magnification 40×, the white boxes indicate zones of higher (63×) magnification, “P” indicates the location of the renal pyramid, scale bar represents 25 µm. * p < 0.05, **p < 0.01.