Fig. 7. HES1 and MYOD1 mark vismodegib-responsive and resistant subsets of cells.

a t-SNE projections as in Fig. 6a, with Gli1⁺ cells indicated. b MALDESI images of cholesterol and vismodegib distribution in sagittal brain sections including both tumor and normal brain. c t-SNE projections as in a with Hes1⁺ cells indicated. d IHC for HES1 and pRB in sagittal hindbrain sections from representative M-Smo mice treated as indicated. e t-SNE projections as in a, with Myod1⁺ cells indicated. f IHC for MYOD1 and pRB in sagittal hindbrain sections from representative M-Smo mice treated as indicated. g Quantification of data from c–f. Dots represent individual replicate mice. Horizontal lines represent means and error bars indicate SEM. h Analysis of MYOD1, pRB and cell cycle phase by flow cytometry. Graphs formatted as in g. i, j Expression microarray data on HES1 and MYOD1 expression in human medulloblastoma samples, as presented in ref. ⁴⁴, with boxes indicating the mean, 25th and 75th percentile and whiskers indicating the 10th and 90th percentiles. Scale bars = 2 mm, except in insets where scale bars = 100 µm. p Values determined by two-sided Student’s t-test.