Fig. 5. Andrographolide attenuated radiation-induced cell death and Caspase-1 activation in lung tissues.

a Representative images of Caspase-1 (stained with FAM-FLICA, shown in green) were analyzed via confocal microscopy. Cell nuclei were visualized by DAPI (blue). Scale bar, 50 μm. b Fluorescence of FAM-FLICA of lung tissues was quantified (five fields per mouse in every group). c Protein was extracted from lung tissues of each group and the expression of Caspase-1 was examined by western blot analysis. Data are expressed as the mean ± S.E.M., n = 6 mice per group, *P < 0.05 vs the IR group.