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. 2019 Dec 20;10:5815. doi: 10.1038/s41467-019-13854-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Bar graph shows local NT-3 expression levels, measured by ELISA, in lumbar cord before and after contusions followed by AAV-NT-3 treatment. Data are presented as mean ± SEM; n = 3–4 mice per each time point; ***P < 0.001 vs baseline (before SCI). One-way ANOVA followed by Tukey’s post-hoc test. b Representative images show synapse-like contacts (arrowheads) between BDA⁺ dPST axons and CTB⁺ lumbar MNs in sham and contusive mice with AAV-NT-3 post-treatment at 2 and 4 wpi. Scale bar = 10 μm. c Quantitative analysis of the number of triple-labeled appositions between BDA⁺ dPST, CTB⁺ MNs, and synaptophysin per each MN per section among three groups. Dots in violin plots represent triple appositions from 3–4 mice in each group. The yellow lines indicate mean ± SD. **P < 0.01, ***P < 0.001; one-way ANOVA followed by Tukey’s post-hoc test. d Representative images show lumbar MN dendritic complexity in the three experimental groups. Curve plot represents sholl analysis of MN morphology among groups. Thick lines represent the average distributions of MN dendrites for each experimental group (n = 3 mice per group); shaded areas represent 95% confidence intervals. Line plot details the difference in the dendritic complexity of MNs at distance from somas between 50 and 160 μm. Data are presented as mean ± SEM. Two-way ANOVA followed by Tukey’s post-hoc test. e Representative images show MN dendritic complexity in sham and T9 contusion mice with AAV-NT-3 pre-treatment and MN dendritic morphology examined at 2 and 4 wpi. Curve plot represents sholl analysis of MN dendritic distribution in the three groups. Thick lines represent the average distributions of MN dendrites among groups (n = 3 mice per group); shaded areas represent 95% confidence intervals. Line plot details the difference in the dendritic complexity of lumbar MNs at distances from somas between 50 and 160 μm. Data are presented as mean ± SEM. Two-way ANOVA followed by Tukey’s post-hoc test. Source data are provided as a Source Data file.