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. 2019 Dec 20;9:19506. doi: 10.1038/s41598-019-55800-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Maximum likelihood tree based on 88 MHC class I exon 3 nucleotide sequences from siskins. One MHC class I sequence (GenBank acc. nr. JN613264) from Falco peregrinus was used as the outgroup. The tree was constructed with PhyML software (version 3.1.2) using the K80 model with gamma distribution and 1000 bootstraps, displaying bootstraps values higher than 700. Four clusters had high bootstrap support which are indicated with bold lines. Green circles represent alleles with high expression, purple circles represent alleles with low expression, white circles represent alleles with undetermined expression and alleles without circles are not expressed. Stars (*) indicates alleles that had a 3 bp insertion. Branch length is unscaled, i.e. all branches are the same length. All non-classical alleles (N = 18) are found in one cluster with strong bootstrap support (987).