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. 2019 Dec 20;9:19482. doi: 10.1038/s41598-019-55736-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Correlation between root relative expression of AtHMA3 and Zn-related genes in transgenic lines. PEX4 and EF1-α4 were used as internal normalization standard across all samples and the expression levels were calculated by 2^−ΔCT method⁵². The points in Fig. 2 refer to individual plants from the three complementation lines used in this work. Significant correlations (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001) are shown as black diamonds (a) and non-significant correlations are shown as grey diamonds (b).