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. 2019 Dec 20;10:5828. doi: 10.1038/s41467-019-13782-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a EM of cells expressing GFP-FBP17. FBP17 signal (marked with arrows) is mostly located around caveolar clusters. Arrows indicate the presence of GFP-FBP17. Mito mitochondria, PM plasma membrane, Cav caveolae, End endosome, ER endoplasmic reticulum, MT microtubules, CCV clathrin-coated vesicle. Scale 1 μm. b Localization of endogenous FBP17 by I-EM. FBP17 (gold signal) in caveolae and in rosettes with different caveolar density are shown. Scale bar 50 nm. c Quantification of the fraction of caveolar structures with gold signal associated with FBP17 antibody. N = 11 (left) and 4 (right) biologically independent cells from 1 experiment. Data represent mean ± S.E.M. Statistical analysis with a two-tailed unpaired t test. ***P < 0.005. d Localization of gold particles associated with endogenous FBP17 in caveolae. Scale bar 50 nm. e Fraction of gold particles associated with endogenous FBP17 in the indicated regions of rosettes.