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. 2019 Dec 20;9:19556. doi: 10.1038/s41598-019-55987-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glucose intolerance. Male C57BL/6J mice were put on experimental diets at the age of 6 weeks for another 12 weeks. Panel A shows the fasting glucose levels in the 12th week of feeding. n = 15 (SCD and CAF) or 20 (NFD and HFD), respectively. Panel B shows the plasma insulin concentration in the 12th week of feeding. n = 6 (SCD, NFD and HFD) or 5 (CAF), respectively. Panel C shows the results of GTT at the end of the experiment after overnight fasting. n = 5 (SCD, CAF and HFD) and 9 (NFD), respectively. Panels E shows the results of ITT (1 IU/kg body weight insulin was injected intraperitoneally) in the 12^th week of experimental feeding after overnight fasting. n = 10 (SCD and NFD) and 9 (CAF and HFD), respectively. The area under the curve (AUC) of GTT and ITT are shown in panels D and F, respectively. Significances marked with ^*,# and ⁺refer to comparisons to the SCD, CAF and NFD group, respectively. ^*,#,+P < 0.05, ^**,##P < 0.01, ^***,###,+++P < 0.001, ns, not significant.