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. 2019 Dec 20;9:19556. doi: 10.1038/s41598-019-55987-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phosphorylation of eNOS. Male C57BL/6J mice were put on experimental diets at the age of 6 weeks for another 12 weeks. Phosphorylation of eNOS at Ser1177 (A) and of Akt at Ser473 (B) in aortic PVAT was studied by Western blot analyses. The blots shown are representative of four independent experiments with similar results. Significances marked with ^*,# and ⁺refer to comparisons to the SCD, CAF and NFD group, respectively. ^*,#P < 0.05, ^**,##,++P < 0.01, ***P < 0.001, ns, not significant. n = 12.