Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 20;10:5806. doi: 10.1038/s41467-019-13646-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Transcriptomic analysis of RNA-seq data using a Spearman correlation matrix demonstrates highly correlated gene expression between paired WTPDX and primary tumors in most sample pairs. Sample pairs constituting the lowest quartile of Spearman correlation (r < 0.836, blue regions of matrix) were associated with neoadjuvant chemotherapy and blastemal poor primary tumors that often transitioned to blastemal-rich WTPDX (arrows). b Principal component analysis was performed for paired WTPDX (circles) and primary tumors (squares), normal kidney specimens (diamonds), and pooled fetal kidney RNA (triangles) using RNA-seq data. WTPDX clustered distinctly from primary tumors. PC1 explained 21.6% of variance among samples and was inversely correlated with expression of WT blastemal archetype and kidney development cap mesenchyme genes. This increased expression of blastemal genes in WTPDX corresponded to increased percent blastema detected on histologic analysis (heatmap display of percent blastema per specimen shown). PC2 explained 10.5% of variance among samples and was positively correlated with expression of WT epithelial and kidney epithelial genes. KT—xenografts, PT—primary tumor, NK—normal kidney, FK—pooled fetal kidney RNA.