Figure 1.
Alignment of the amino acid sequence of P. aeruginosa lysyl-tRNA synthetase (LysRS) with homologs. The protein sequences of LysRS from Bt, Burkholderia thailandensis; Ec, E. coli; Pa, P. aeruginosa; and Tth, T. thermophilus were downloaded from the National Center for Biotechnology Information. Accession numbers for LysRS protein sequences of B. thailandensis, E. coli (S), E. coli (U), P. aeruginosa, and T. thermophilus are AOJ57238, AAA83071, Q8FAT5, NP_252390, and P41255, respectively. Sequence alignments were performed using Vector NTI Advance 11.5.4 (Invitrogen). Identical residues are indicated by white letters on a black background, whereas similar sequences are black letters on a gray background. The three structural motifs (1, 2, and 3) are indicated. Amino acids that interact with adenosine triphosphate (●), lysine (◼), and tRNALys (◆) are indicated above the aligned residues.