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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: SLAS Discov. 2019 Sep 9;25(1):57–69. doi: 10.1177/2472555219873095

Figure 6.

Figure 6.

Toxicity of the hit compounds in cultures of human cell lines. Toxicity of the hit compounds was measured using human embryonic kidney 293 (HEK-293) cell cultures. The compound concentration ranged from 25 to 400 μg/mL. The data points represent the average values for assays carried out in triplicate. “% Positive” indicates the percentage of activity observed relative to activity in assays in which only DMSO was added to the assay in the absence of compound. The curve fits and CC50 values were determined using the sigmoidal dose-response model in XLfit 5.3 (IDBS). (A) BT06F11 was not toxic at any concentration tested. The hatched column represent the growth of cultures in the positive control assays, and the solid column represents the cultures containing the indicated concentration of BT06F11. The CC50 values for (B) BM01D09 and (C) BT08F04 were 370 μg/mL and 61 μg/mL, respectively. The control staurosporine (D) was serially diluted in assays from 1 to 0.001 μg/mL and exhibited a CC50 of 0.003 μg/mL.