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. Author manuscript; available in PMC: 2019 Dec 21.
Published in final edited form as: ACS Chem Biol. 2019 Nov 14;14(12):2793–2799. doi: 10.1021/acschembio.9b00678

Figure 3.

Figure 3.

Incorporation of NCK and NCibK into sfGFP proteins in E. coli. (A) Expression of sfGFP mutants in the presence or absence (−) of 1 mM NCK or NCibK, analyzed by SDS-PAGE. (B) ESI-MS analysis of sfGFP mutants containing NCibK or NCK. Two desired peaks were observed for each sample. One is the full-length sfGFP mutant, and the other one is the full-length sfGFP mutant without N-terminal Met. The 27577 Da peak corresponds to the misincorporation of Phe at 151 position of sfGFP. (C) ESI-MS analysis of NCibK-containing sfGFP mutant reacted with dpTz. The 27995 and 27864 Da peaks correspond to the conjugated products with and without N-terminal Met, respectively.