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. Author manuscript; available in PMC: 2020 Dec 20.
Published in final edited form as: ACS Chem Biol. 2019 Oct 14;14(12):2595–2605. doi: 10.1021/acschembio.9b00482

Figure 1. In Vitro Activity of IRE1β’s RNase Domain.

Figure 1.

A. Schematic of the XBP1 cleavage assay with IRE1α* and IRE1β*. An increase in fluorescence is observed upon cleavage of a FRET-quenched XBP1 mini-substrate. B. Real time fluorescence curves for 75 nM IRE1α* and 75 nM IRE1β* with 250 nM of the XBP1 mini-substrate. C. Michaelis-Menten curves of IRE1α* and IRE1β* for the XBP1 mini-substrate. Data shown are mean ± SEM, n=3.