Table 1: Different technologies for studying Replication-Coupled Nucleosome Assembly (RCNA).

SuperResolved Chromatin Fibers (SRCF) [18]; Sister Chromatids After Replication by DNA sequencing (SCAR-seq) [51]; Enrichment and Sequencing of Protein Associated Nascent DNA (eSPAN) [58]; Mapping In Vivo Nascent Chromatin with EdU and Sequencing (MINCE-seq) Nascent Chromatin Avidin Pull-down (NChAP) [60]; Nascent Chromatin Occupancy Profiling (NCOP) [129].

Technique	Sequence information	Single-molecule resolution	Unbiased versus candidate approach	High-throughput	Sister chromatid resolution
SRCF [18]	no	yes	candidate	no	yes
SCAR-seq [51]	yes	no	candidate	yes	yes
eSPAN [58]	yes	no	candidate	yes	yes
MINCE-seq [128]	yes	no	unbiased	yes	no
NChAP [60]	yes	no	unbiased	yes	yes
NCOP [129]	yes	no	unbiased	yes	no