Extended Data Figure 3. The diauxic lag disappears in the supplementation regime.

a. Diauxic growth curves (+) of the titratable PtsG strain (NQ1243) in medium with 1.7 mM glucose and saturating glycerol. Each curve is for a different 3MBA concentration (indicated in the figure) and horizontally shifted for convenience. The data shown is from a single series of experiments.

b. Lag times were determined for each diauxic growth curve; the method is illustrated here using the condition [3MBA] = 800 μM as an example. We fitted exponential curves through the two growth phases (green lines) and a horizontal line through the lag phase (horizontal black line). The lag time (indicated in gray) is heuristically defined as the horizontal distance between the intersections of the green lines with the horizontal one. In this case, a lag time of 46 minutes is found.

c. Lag times of the growth curves versus [3MBA]. The diauxic lag time vanishes precipitously when [3MBA] is tuned below 100 μM, where glycerol and glucose are co-utilized.