Figure 1.
High-throughput screen for identifying inhibitors of HPV infection. Shown is a schematic illustrating the basic steps in the HTS. See Materials and Methods for additional details. Briefly, HPV16-PsVs containing the Renilla reporter genes are analyzed for their ability to infect as judged by the levels of luminescence. Cell viability was assessed in parallel 384-well plates. In the specific HTS carried out in this study, the compounds were added at a 8.3 μM/0.3% DMSO final concentration 2 h prior to infection.