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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Eur J Pharmacol. 2019 Nov 13;865:172795. doi: 10.1016/j.ejphar.2019.172795

Fig. 1. Nlrp3 deficiency prevents nicotine-induced tight junction disruption in coronary arterial endothelium of mice.

Fig. 1.

Nlrp3+/+ or Nlrp3−/− mice were treated with nicotine (i.p., 2 mg/kg/d) or vehicle control (PBS) as described in Methods. (A) Fluorescent images show alexa-555-ZO-1 (red) in coronary arteries. The endothelium was visualized by alexa-488-vWF staining (green). The summarized data on the right panel show the fluorescence intensity ratio of ZO-1 over vWF (n=5-7 mice). (B) Fluorescent images show alexa-555-ZO-2 (red) in coronary arteries. The endothelium was visualized by alexa-488-vWF staining (green). The summarized data on the right panel show the fluorescence ratio of ZO-2 over vWF (n=5-8 mice). Each image also includes an image for the area of interest (AOI). Data are presented as means ± S.D. *P < 0.05 vs. Nlrp3+/+ Ctrl; # P < 0.05 vs. Nlrp3+/+ with nicotine. Scale bar = 50 μm.