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. Author manuscript; available in PMC: 2020 Oct 30.
Published in final edited form as: J Am Chem Soc. 2019 Oct 22;141(43):17068–17074. doi: 10.1021/jacs.9b08677

Figure 7:

Figure 7:

(A) Synthesis of MAGL reactive probe 9. (B) Live cells were treated with probe 9 for 1 h, followed by 2 μM TCO-TAMRA for 30 min, cell lysis, and analysis by in-gel fluorescence (C) In-gel fluorescence signals for a dose response of probe 9. Probe 9 (32 nM, 1 h) was competed by pre-treatment with MAGL inhibitor KML29 (300 nM, 1 h). (D) KML29 also incorporates a HFIP carbamate warhead. (E) Dose response fitting of the fluorescence signals of MAGL normalized by the total protein amount indicated by Coomassie staining. Data are reported as mean ± SEM (n = 2). See Fig S-21 for Coomassie staining.