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. 2019 Dec 19;3(1):e201900476. doi: 10.26508/lsa.201900476

Figure S5. Effect of Ubp2 and Cdc48 in protein stability of Fzo1K398R.

Figure S5.

(A) Stability of genomically HA-tagged Fzo1 or Fzo1K398R in wt or ∆ubp2 cells. Cells as in Fig 3B, but with adjusted HA-Fzo1K398R levels by additional ectopic expression, were treated and analysed as in Fig 2A. (B) Stability of HA-tagged Fzo1 and FzoK398R in wt and cdc48-2 cells. (A) Cells as in Fig 3E were subjected to a cycloheximide chase as in (A). The samples were analysed by SDS–PAGE and Western blot using an antibody against HA. PoS, Ponceau S.