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. 2019 Oct-Dec;11(4):270–276.

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Copyright© 2019 Avicenna Research Institute

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Annexin V apoptosis assay in CLL and healthy PBMCs. A) Box plot represents the frequency (%) of apoptotic cells induced by2D8 mAb in comparison with isotype control mAb (negative control) or staurosporine (positive control) in CLL patients (n=6) (black color) or healthy individuals (n=6) (gray color). Boxes show values between the 25^th and 75^th percentiles. The horizontal line within the box represents the median value. B) Dot plot diagram represents apoptosis induction in one CLL PBMC in comparison with one healthy PBMC. To calculate the percentage of apoptotic cells, the amount of cell percentage in early (i.e. Annexin V+, PI−) and late (i.e. Annexin V+, PI+) stages of apoptosis was added together. ** means p≤0.01. ns stands for not significant.