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. 2019 Dec 20;17:7. doi: 10.1186/s12953-019-0155-y

Table 1.

Proteomic studies in the case of FSGS, MGN and IgAN

CKD type Sample Type Method Putative Biomarkers Ref.
MGN Tissue MALDI-MS imaging sonic Hedgehog (SHH), α-smooth muscle actin (α-SMA) (Smith et al., 2019)
Urine tandem mass tag (TMT), nano-scale LC-MS/MS Alpha-1-antitrypsin, Serotransferrin, Ceruloplasmin, Serum albumin, Carbonic anhydrase 1, Transthyretin, Haptoglobin, Leucine-rich alpha-2-glycoprotein, Alpha-1B-glycoprotein, Afamin (Pang et al., 2018)
Serum, Tissue 2DE, semidry blotting, Mass α-enolase, superoxide dismutase 2, vimentin, aldolase reductase, glycine-tRNA ligase, elongation factor 2 (Bruschi et al., 2015)
Urine 2DE, MALDI-TOF-MS progesterone-induced blocking factor 1 isoform a, ribosomal protein L5, vimentin, tropomyosin isoform 6, α-1-antitrypsin, serotransferrin precursor, Ba1–647, haptoglobin precursor, preprohaptoglobin, serum albumin precursor, CTP:phosphocholine cytidylyl transferase, anti-idiotype immunoglobulin M light chain, vascular endothelial growth factor A splice variant VEGF 102 (Ngai et al., 2006)
Serum SDS-PAGE 29 KD, 5 KD (Pant et al., 2016)
Tissue iTRAQ, MALDI-TOF-TOF-MS C1RL, TUBB, CD74, CORO1A, B2M, KRT1, ARHGDIB, HLA-DRB1, HLA-A, HLA-DRB1, IGHG1, KV102, ERAP1, MYLPF, GBP1, HLA-B, FTH1, VCAM1, C4BPA, HSPD1, C1QBP (Sui et al., 2015)
Interstitial Fluid, Tissue, Urine, Serum, Podocyte Cell Lines ESI-Q-TOF-MS, immunofluorescence PDLI5, LDB3 (Ligabue et al., 2013)
Urinary Microvesicles, Tissue iTRAQ labeling and LC-MS/MS, immunofluorescence LIMP2, TPP1, AMBP, PDZK1P1, DCHS2, MGAM, SLC9A3R1, ATAD5, HYDIN, MYO15A, C7, MUC1, SDCBP, GPRC5B, ATG2B, RAC1, EHMT1, RHOC, KMT2B, EPS8, EVC2, SLC44A2, SLC12A3, MYO9B, CCDC81 (Rood et al., 2015)
Tissue MALDI-MS imaging serine/threonine protein kinase MRCK gamma, PLA2R, IgG4 (Smith et al., 2017)
IgAN Tissue immunostaining CAP1, PRCP, SHC1 (Krochmal et al., 2017)
Urine IEF/LC-MS/MS AFM, ALB, APOA1, AZGP1, A2M, CA1, CP, C3, C4A, FN1, F2, GC, HP, IGFBP7, PSAP, SERPINA1, SERPINA3 (Mucha et al., 2014)
Urine nanoscale LC-high resolution tandem MS transferrin, α1-antitrypsin, albumin fragments, fibulin-5, prasoposin, YIP1 family member 3, osteopontin (Mohammadi Majd et al., 2018)
Urine 2D-LC-MS/MS, iTRAQ, Western blot ICAM1, TIMP1, SERPINC1, ADIPOQ (Guo et al., 2018)
Urine LC-MALDI-TOF/MS, proteins arrays, immunomagnetic isolation IgA-uromodulin complex, α1-antitrypsin, galactose-deficient IgA1 (Neprasova et al., 2016)
Tissue MALDI-MS imaging m/z of 4963, 5072 (Smith et al., 2016)
Urine nano-LC-MS/MS CD44, glycoprotein 2, vasorin, protocadherin, epidermal growth factor, CLM9, dipeptidyl peptidase IV, NHL repeat-containing protein 3, utreoglobin, SLAM family member 5 (Samavat et al., 2015)
Urine SELDI-TOF-MS, MALDI-TOF-MS perlecan laminin G-like peptide, IgK light chains (Rocchetti et al., 2013)
Urine, Serum, Plasma magnetic bead technology, MALDI-TOF-MS uromodulin, bradykinin and α-1-antitrypsin urine m/z:1769, 1898, 1913, 1945, 2491, 2756, 2977, 3004, 3389, 4752 blood m/z:2953, 5337, 9287, 9289 (Graterol et al., 2013)
Urine MALDI-TOF-MS m/z: 2740, 3038, 2747, 3032, 3235, 2095, 3328, 6174, 6189, 2754 (He et al., 2012)
Urine 2DE, nano-HPLC-ESI-MS/MS kininogen, inter-α-trypsin-inhibitor heavy chain 4, tranthyretin (Rocchetti et al., 2008)
Urine 2DE, MALDI-TOF-MS A panel of 59 protein markers (Park et al., 2006)
Urine 2D-DIGE, LC-MS/MS α1-microglobulin, transferrin, albumin, retinol-binding protein 4, β-globin, carbonic anhydrase I, cystatin C, α2-glycoprotein 1 (Yokota et al., 2007)
Urinary Exosomes LC-MS/MS aminopeptidase N, α-1-antitrypsin, vasorin precursor, ceruloplasmin (Moon et al., 2011)
Urine 2DE, MALDI-TOF-TOF/MS, western blot α-1-antitrypsin, albumin fragments, α-1-β-glycoprotein, laminin G-like fragment of endorepellin (Surin et al., 2013)
Urine CE-ESI-TOF-MS A panel of polypeptides molecular weights (Haubitz et al., 2005)
Urine nanoflow LC-MS/MS, GeLC-MS/MS afamin, leucine-rich alpha-2-glycoprotein, ceruloplasmin, alpha-1-microgolbulin, hemopexin, apolipoprotein A-I, complement C3, vitamin D-binding protein, beta-2-microglobulin, retinol-binding protein 4 (Kalantari et al. 2013)
FSGS Urine nano-flow LC-MS/MS RNAS2, CD59, PTGDS, B2MG, AMBP, SULF2, CBG, AFAM, MXRA8, CO6A1, ACTG, HPT (S. Kalantari et al., 2014)
Urine LC-MS/MS, Western blot serum albumin, serotransferrin, α-1-1antiproteinase, afamin, ceruloplasmin, plasminogen, AMBP (Zhao et al., 2014)
Urine magnetic bead-based technology, MALDI-TOF/MS Uromodulin, α-1-antitrypsin fragment (Perez et al., 2014)
Urine nano-LC-MS/MS apolipoprotein-A1, matrix-remodeling protein 8 (Shiva Kalantari et al., 2014)
Urine 2D-DIGE, MALDI-TOF MS α-1-antitrypsin, transferrin, 39S ribosomal protein L17, histatin-3, calretinin (Perez et al., 2017)
Urine 2DE, MALDI-TOF/MS glutathione S-transferase, collagen IV fragment, E-cadherin (Shui et al., 2008)
Urine 2DE, tandem-MS UBA52, CD74, SKP1, CXADR, S100A13, SYNE1, PVALB, FRY, GSTA3, CROCC, LRBA, DAB2 (Wang et al., 2017)
Serum SDS-PAGE 29 KD, 5 KD (Pant et al., 2016)
Tissue MALDI-MS imaging m/z of 4025, 4048 (Smith et al., 2016)
Urine nano-flow LC-MS/MS CD59, CD44, IBP7, DPEP1, Robo4 (Nafar et al., 2014)

MGN Membranous glomerulonephritis, IgAN Immunoglobulin A nephropathy, FSGS Focal segmental glomerulosclerosis, MCD Minimal change disease, iFSGS idiopathic Focal segmental glomerulosclerosis, iMGN idiopathic Membranous glomerulonephritis, MPG Membranoproliferative glomerulonephritis, DN Diabetic nephropathy, LN Lupus nephritis, TBMN Thin-basement membranous nephropathy