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. 2019 Dec 21;24:79. doi: 10.1186/s12199-019-0835-3

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Increased PD-L1 expression level in OS tissues. a Immunohistochemical assay was performed to detect the expression of PD-L1 in OS and OS-adjacent tissues (× 400 magnification), the red arrow represents positive PD-L1 staining. b RT-qPCR was performed to detected the mRNA level of PD-L1 in OS and OS-adjacent tissues (n = 6). The results were presented as the mean ± standard deviation. **P < 0.01. PD-L1, programmed death-ligand 1; OS, osteosarcoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction