Fig. 2.

Detection of lentivirus transfection efficiency in K7M2 cells. K7M2 cells were transfected with the lentivirus carried both of GFP or PD-L1 gene for 72 h. a Images of K7M2 cells were captured using a fluorescent microscope following transfection with lentivirus (× 100 magnification). b RT-qPCR assay was applied to detect the PD-L1 mRNA expression level. *P < 0.05. The experiment was repeated three times, and results are presented as the mean ± standard deviation. GFP, green fluorescent protein; PD-L1, programmed death-ligand 1