Table 1.
Primers used for ARMS assays and DNA sequencing to genotype four VDR polymorphisms
| SNPs | Sequences | Genotype-based product size |
|---|---|---|
| TaqI (rs731236) | ||
| Outer$ | F: 5′ GCT GCC GTT GAG TGT CTG TGT GGG TG 3’ | |
| R: 5′ ACA AGG GGC GTT AGC TTC ATG CTG CAC TC 3’ | TT: 415 and 300 bp | |
| Inner | T/F: 5′ CAG GAC GCC GCG CTG CTT 3’ | TC: 415, 300 and 150 bp |
| C/R: 5′ CGG TCC TGG ATG GCC GCG 3’ | CC: 415 and 150 bp | |
| BsmI (rs1544410) | ||
| Outer$ | F: 5′ GTG GTG TGT GGA CGC TGA GGT G 3’ | |
| R: 5′ TTC CTT GAG CCT CCA GTC CAG GAA AG 3’ | TT: 458 and 350 bp | |
| Inner | T/F: 5′ GGG CCA CAG ACA GGC CTA CA 3’ | TC: 458, 350 and 150 bp |
| C/R 5′ CAG AGC CTG AGT ATT GGG AAC GC 3’ | CC: 458 and 150 bp | |
| ApaI (rs7975232) | ||
| Outer$ | F: 5′ ATG GAA GGA CCT AGG TCT GGA TCC TAA ATG C 3’ | |
| R: 5′ GCT GCA CTC AGG CTG GAA GGA G 3’ | TT: 420 and 280 bp | |
| Inner | T/F: 5′ GTG GTG GGA TTG AGC AGT GAA GT 3’ | GT: 420, 280 and 180 bp |
| G/R: 5′ ACA GGA GCT CTC AGC TGG ACC 3’ | GG: 420 and 180 bp | |
| FokI (rs2228570) | ||
| Outer$ | F: 5′ ATG CCC ACC CTT GCT GAG CTC 3’ | |
| R: 5′ ATC TGG AGC TGA GAG GAG GGA AAA GAA GA 3’ | GG: 477 and 360 bp | |
| Inner | G/F: 5′ GCC TGC TTG CTG TTC TTA CAG GAA C 3’ | GA: 477, 360 and 160 bp |
| A/R: 5′ CTG GCC GCC ATT GCC TTC A 3’ | AA: 477 and 160 bp | |
($) Outer primers were used for direct sequencing to genotype VDR polymorphisms