KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Beta-Actin, Mouse clone AC-15 | Sigma-Aldrich | Cat # A1978; RRID: AB_476692 |
| CD4, Mouse Clone RPA-T4 (APC) | BD Biosciences | Cat # 555349; RRID: AB_398593 |
| HA.11 Epitope Tag antibody (Alexa Fluor® 647) | BioLegend | Cat # 682404; RRID:AB_2566616 |
| HIV-1 Core Antigen, Mouse clone KC57 (RD1) | Beckman Coulter | Cat # 6604667 |
| Human HLA-A2 Antibody, Mouse clone BB7.2 (R-PE) | BD Biosciences | Cat # 558570; RRID: AB_647220 |
| Mouse IgG, Goat polyclonal, minimal x-reactivity (HRP) | BioLegend | Cat # 405306; RRID: AB_315009 |
| Nef, Rabbit polyclonal | NIH AIDS Reagents Program, gift of R Swanstrom (Shugars et al., 1993) | Cat # 2949 |
| Rabbit IgG, Donkey polyclonal, minimal x-reactivity (HRP) | BioLegend | Cat # 406401; RRID: AB_2099368 |
| SERINC5, Rabbit polyclonal | Abcam | Cat # ab204400 |
| Bacterial and Virus Strains | ||
| E. cloni 10G SOLOs Chemically Competent Cells | Lucigen | Cat # 0106-2 |
| One Shot Stbl3 Chemically Competent E. coli | Invitrogen | Cat # C737303 |
| Biological Samples | ||
| Human Peripheral Blood Mononuclear Cells, Frozen | Stem Cell Technologies | Cat # 70025 |
| HIV-1 subtype B Nef clones | Mwimanzi et al., 2013 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Lectin from Phaseolus vulgaris Erythrohemagglutinin (PHA-E) | Sigma-Aldrich | Cat # L8629 |
| Human Recombinant IL-2 | StemCell Technologies, Inc. | Cat # 78036 |
| Fixation/Permeabilization Solution Kit | BD Biosciences | Cat # 554714 |
| DNAfectin 2100 Transfection Reagent | Applied Biological Materials | Cat # G2100 |
| TrypLE Express Enzyme (1X), phenol red | GIBCO | Cat # 12605010 |
| Critical Commercial Assays | ||
| Gene Pulser MXcell Electroporation System | Bio-Rad | Cat # 165–2670 |
| 96-Well Electroporation Plates | Bio-Rad | Cat # 1652681 |
| HIV-1 p24 ELISA Kit | XpressBio | Cat # XB-1000 |
| Steady-Glo® Luciferase Assay System | Promega | Cat # E2550 |
| Deposited Data | ||
| HIV-1 subtype B Nef sequences | Mwimanzi et al., 2013 | GenBank accession JX171199-JX171243 (controllers) and JX440926-JX440971 (progressors) |
| Experimental Models: Cell Lines | ||
| CEM-A*02, human T cell | Anmole et al., 2015 | N/A |
| HEK293T, human kidney cell | American Type Culture Collection | Cat # CRL-3216 RRID: CVCL_0063 |
| Jurkat LTR-GFP CCR5+ (JLTRG-R5), human T cell | NIH AIDS Reagents Program, gift of O Kutsch (Kutsch et al., 2004) | Cat # 11586 |
| Jurkat LTR-GFP CCR5+ (JLTRG-R5) SERINC5 knockout, human T cell | This paper | N/A |
| TZM-bl, human carcinoma cell, derived from HeLa | NIH AIDS Reagents Program, gift of JC Kappes and X Wu (Derdeyn et al., 2000; Platt et al., 1998; Wei et al., 2002) | Cat # 8129-442; RRID: CVCL_B478 |
| Oligonucleotides | ||
| Nef_Forward Primer (AGAGCACCGG CGCGCCTCCA CATACCTASA AGAATMAGAC ARG) | This paper | N/A |
| Nef_Reverse Primer (GCCTCCGCGG ATCGATCAGG CCACRCCTCC CTGGAAASKC CC) | This paper | N/A |
| Recombinant DNA | ||
| pHEF-VSVG | NIH AIDS Reagent Program, gift of L-J Chang (Chang et al., 1999) | Cat # 4693 |
| pMaxFP-Green-N | Amaxa | Cat # VDF-1012 |
| pNL4-3, infectious HIV-1 molecular clone | NIH AIDS Reagents Program, gift of M Martin (Adachi et al., 1986) | Cat # 114 |
| pSELECT-GFPzeo-mcs | InvivoGen | Cat # psetgz-mcs |
| pSELECT-ΔGFP-zeo-mcs | This paper | N/A |
| pX330-U6-Chimeric_BB-CBh-hSpCas9 | Addgene, provided by F Zhang (Cong et al., 2013; Ran et al., 2013) | Cat # 42230; RRID: Addgene_42230 |
| Software and Algorithms | ||
| FlowJo 9.9.5 | FlowJo, LLC | FlowJo |
| Prism 7 | Graphpad | Prism |