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. Author manuscript; available in PMC: 2020 Jun 2.
Published in final edited form as: Nat Chem. 2019 Dec 2;12(1):71–75. doi: 10.1038/s41557-019-0370-2

Table 1.

Reaction Optimization

graphic file with name nihms-1541076-t0004.jpg
entry X LED Irradiation yield 2 (%)a e.r. b yield 3 (%)a
1c Br none 18 89:11 37
2 Br Cyan LED 78 80:20 19
3 Cl Cyan LED 94 95:5 4
4 Cl none - - -
5d Cl Cyan LED - - -

Standard Reaction Conditions. Substrate (10 mM), OPR1 (50 μM), tricine buffer (100 mM, pH = 8.0), 10 (v/v)% IPA (iPrOH), Cyan LEDs, 24 h.

a

Yield determined via reverse phase HPLC relative to an internal standard.

b

Enantiomeric ratios determined via HPLC on a chiral stationary phase.

c

NADP+ (1 mol %), Glucose (1 equiv) and GDH-105 (glucose dehydrogenase) added.

d

No enzyme added.