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CpGH84s kinetic parameters using para-nitrophenyl-β-D-N-acetyl-glucosamine (pNP-GlcNAc) as a substratea
| GH84A | GH84B | GH84C | GH84D | |
|---|---|---|---|---|
| Km (mM) | 0.65 ± 0.05 | 4.85 ± 0.32 | 0.13 ± 0.01 | 2.23 ± 0.18 |
| V max (μM min−1) | 1.37 ± 0.03 | 9.85 ± 0.40 | 6.98 ± 0.09 | 3.63 ± 0.13 |
| k cat (s−1) | 0.72 ± 0.00 | 5.50 ± 0.22 | 3.83 ± 0.06 | 2.00 ± 0.06 |
| k cat/Km (mM−1 s−1) | 1.11 ± 0.09 | 1.13 ± 0.09 | 29.46 ± 2.31 | 0.90 ± 0.08 |
indicates standard errors calculated from experiments done in triplicate.
