Table 1.
Primers, fragment patterns, and enzymes used for RFLP analysis.
| Polymorphisms | Primers (5′–3′) | Amplicon sizes (bp) | Restriction enzymes |
|---|---|---|---|
| -2518A/G (rs1024611) |
F: GCTCCGGGCCCAGTATCT R: ACAGGGAAGGTGAAGGGTATGA |
182,54 236 |
PvuII |
|
| |||
| 362C/G (rs2857656) |
F: CTAGGCTTCTATGATGCTAC R: TCCATTCACTGCTGAGACCA |
201,110 311 |
Hpy188I |
|
| |||
| CCL2I/D (rs3917887) |
F: GCTGATCTTCCCTGGTGCTGAT R: CATTAAATCCCAGTGCTTCTGCCTA |
I:202 D:188 |
— |
F: forward primer; R: reverse primer; I: insertion; D: deletion. In order to ratify the data generated by the PCR-RFPL method, we analyzed 25% of the samples randomly.