Figure 4.

A schematic representation of the assay procedure where total RNA was extracted from the specimen and the bacterial 16S rRNA was amplified by asymmetric real time-PCR. Single-strand DNA of the amplified product was then captured by beads conjugated to capture probes, before hybridizing with magnetic nanoparticles (MNPs) to form a magnetic sandwich complex. Samples were subsequently analyzed using a μNMR system. Reproduced from Chung et al. [84] with a Copyright Clearance Center's RightsLink® service and order number 4658140169987.