Table 1.
Primers | Sequence (5′–3′) |
---|---|
For reverse-transcription PCR | |
miR-25-3p-RT | GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCAGAC |
U6-RT | CGCTTCACGAATTTGCGTGTCAT |
| |
For quantitative real-time PCR analysis | |
SNHG5-F | AAGCTTCTTTTACGTCGGCCTTCGCGAGCGTCTGG |
SNHG5-R | GGATCCTCGAGTTAGTGGATTTTCCATTTAATGCTCC |
miR-25-3p-F | GGCCATTGCACTTGTCTCGGTCTGA |
miR-25-3p-R | CAGTGCGTGTCGTGGAGT |
BTG2-F | CATCATCAGCAGGGTGGC |
BTG2-R | CCCAATGCGGTAGGACAC |
U6-F | GCTTCGGCAGCACATATACTAAAAT |
U6-R | CAGTGCGTGTCGTGGAGT |