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. 2019 Dec 9;2019:5320902. doi: 10.1155/2019/5320902

Table 1.

Sequences and binding temperatures for RT-qPCR (temp).

Gene Sequence fwd (5′ ⟶ 3′) Sequence rev (5′ ⟶ 3′) Temp (°C)
GADPH ATGACGAGGGCCTGGAGTGTG CCTATGTGCTGGCCTTGGTGAG 60
VEGF ATGACGAGGGCCTGGAGTGTG CCTATGTGCTGGCCTTGGTGAG 60
TGF-β1 GCGTGCTAATGGTGGAAAC CGGAGCTCTTGATGTGTTGAAGA 60
PEDF AGTTACGAAGGCGAAGTCACCAAGTC GCCCGGTGTTCCACCTGAGTC 50