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. 2019 Dec 7;2019:2193019. doi: 10.1155/2019/2193019

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Copyright © 2019 Huiqin Luo et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

I1PP1 overexpression alleviated CaMKII oxidation and phosphorylation in cardiomyocytes after H/R injury. After infection of the I1PP1 recombinant adenovirus, the cardiomyocytes were subjected to hypoxia for 4 h followed by 12 h reoxygenation. (a) Expressions of CaMKII oxidation (ox-CaMKII) and total CaMKII in cardiomyocytes after H/R injury were quantified by western blot. GAPDH was used as a loading control. (b) Expression of CaMKII phosphorylation (p-CaMKII) and total CaMKII in cardiomyocytes after H/R injury was quantified by western blot. GAPDH was used as a loading control. Plots represent the mean ± SEM; n = 6. Statistical significance: ^∗∗P < 0.01 compared with the control; ^##P < 0.01 compared with H/R.