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. 2019 Dec 21;16:44. doi: 10.1186/s12977-019-0507-9

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© The Author(s) 2019

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Characterization of HTLV-1 proviral clones. An HTLV-1 LTR-luciferase assay and ELISA specific for HTLV-1 structural protein p19 Gag were performed as in vitro functional assays. HEK293T cells were co-transfected with an LTR-1-Luc reporter construct and 1 µg of HTLV-1, HTLV-1∆CTCF, HTLV-1p12Stop, or empty (pcDNA3) plasmids. 48 h post-transfection cells and supernatant were collected for LTR-transactivation luciferase assay a and p19 Gag ELISA b, respectively. HTLV-1, HTLV-1∆CTCF, HTLV-1p12Stop and proviral clones generated comparable LTR-transactivation and p19 Gag production