Fig. 4.

HTLV-1 CTCF binding site is dispensable for early in vivo viral persistence. rPBMC genomic DNA was isolated at 0, 2, 4, 8, and 12 weeks post-infection and subjected to probe-based qPCR using the Gag/Pol primer and probe set described in Table 2. Each symbol represents the copy number of a single inoculated rabbit at 0, 2, 4, 8, or 12 weeks post-infection within each group. Bars represent mean copy number per cell. There was no significant difference in proviral load between HTLV-1 or mutant inoculated rabbits. A mixed model analysis with a Bonferroni correction was performed in weeks 8 and 12 to determine statistical significance. A p < 0.0083 was considered a statistically significant change