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. 2019 Dec 21;16:162. doi: 10.1186/s12985-019-1271-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Infectious activity of assembled viruses featuring different NS RNA structures after their rescue by the reverse genetics technique

Viruses are: “1–1” - hairpin in regions 1 and 2; “1–0” - hairpin at region 1; “0–1” - hairpin at region 2; and “0–0” - without hairpins. Assembled virus titers were determined 72 h post transfection in Vero cell culture. Error bars represent mean ± s.d (n = 3 in two independent experiments). Differences were analyzed by one-way ANOVA with Tukey’s Multiple Comparison Test