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. 2019 Dec 21;16:162. doi: 10.1186/s12985-019-1271-0

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — NS1 expression in cells infected by assembled viruses featuring different NS RNA structures. MDCK (a) or A549 (b)cell cultures were infected with virus at moi = 10 TCID₅₀/cell. Infected cells were fixed at the indicated time points, and NS1 protein levels were analyzed by ELISA. Error bars represent mean ± SD. Differences were analyzed by two-way ANOVA with Tukey’s multiple comparisons test (**p < 0.001, ****p < 0.00001)