Table 4.
Urinary proteomic biomarkers in Wistar (n = 8) and HHTg rats (n = 8) observed at 3 months of age before microalbuminuria onset and after microalbuminuria manifestation at 20 months of age.
| 3 m | P 3 m value | 20 m | P 20 m value | P W/HHTg value | |||
|---|---|---|---|---|---|---|---|
| W | HHTg | W | HHTg | ||||
| Malondialdehyde (ng/ml) | 23.3 ± 0.7 | 25.4 ± 1.5 | n.s. | 25.0 ± 0.9 | 27.8 ± 1.2 | n.s. | ∗ |
| 8-Isoprostan (pg/ml) | 22.8 ± 1.6 | 26.2 ± 1.6 | n.s. | 22.6 ± 1.6 | 25.3 ± 1.6 | n.s. | n.s. |
| 4-Hydroxynonenal (ng/ml) | 15.7 ± 1.0 | 19.8 ± 0.6 | ∗ | 16.0 ± 1.9 | 21.2 ± 0.6 | n.s. | ∗∗ |
| IL-6 (pg/ml) | 48.7 ± 1.4 | 82.3 ± 4.2 | ∗∗∗ | 53.7 ± 0.7 | 81.3 ± 4.8 | ∗∗∗ | ∗∗∗ |
| IL-8 (pg/ml) | 20.9 ± 1.7 | 44.9 ± 1.6 | ∗∗∗ | 25.9 ± 1.6 | 44.6 ± 1.8 | ∗∗∗ | ∗∗∗ |
| MCP-1 (ng/ml) | 1.7 ± 0.1 | 3.4 ± 0.2 | ∗∗∗ | 2.0 ± 0.1 | 3.3 ± 0.2 | ∗∗∗ | ∗∗∗ |
| EGF (ng/ml) | 4.7 ± 0.3 | 2.0 ± 0.2 | ∗∗∗ | 4.8 ± 0.2 | 2.0 ± 0.1 | ∗∗∗ | ∗∗∗ |
| α1-Antitrypsin (ng/ml) | 12.1 ± 0.7 | 22.0 ± 1.3 | ∗∗∗ | 14.1 ± 1.0 | 21.7 ± 1.5 | ∗∗∗ | ∗∗∗ |
| Heparan sulfate (μg/ml) | 0.07 ± 0.01 | 0.28 ± 0.01 | ∗ | 0.13 ± 0.01 | 0.30 ± 0.01 | ∗ | ∗∗∗ |
Values are given as mean ± SEM; n = 8 for each group; ∗ denotes P < 0.05, ∗∗ denotes P < 0.01, and ∗∗∗ denotes P < 0.001. P3 m: probability reflecting the effect of the strain at 3 months of age and were analysed by two-way ANOVA and by the Bonferroni post hoc test. P20 m: probability reflecting the effect of the strain at 20 months of age and were analysed by two-way ANOVA and by the Bonferroni post hoc test. PW/HHTg: probability reflecting the effect of the strain and were analysed by two-way ANOVA and by the Bonferroni post hoc test. IL-6: interleukin 6, IL-8: interleukin 8, MCP-1: monocyte chemoattractant protein-1, EGF: epidermal growth factor.